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. Author manuscript; available in PMC: 2020 May 24.
Published in final edited form as: J Vis Exp. 2019 Nov 13;(153):10.3791/60365. doi: 10.3791/60365

Table of Materials

Name of Material/Equipment Company Catalog Number Comments/Description
3-port manual valves LabSmith Manual 3-Port Selector Valve (MV201-C360) https://products.labsmith.com/mv201-manual-3-port-selector-valve/#.XNYEC9NKh26
Cannulae Vita Needle Company 304 Stainless steel tubing, Outer Diameter 228μm, Inner Diameter 165μm https://www.vitaneedle.com/assets/files/Vita_Needle_Master_Tubing_Gauge_Chart.pdf
Cleaving stone Molex Cleaving stone 1” x 1” (part No. 1068680064) Highly recommended to follow method for cleaving capillary tubing: https://www.cmscientific.com/info_sheets/cleaving_procedure.pdf
Clorhexidine diacetate Walmart Nolvasan solution disinfectant (AAP311) Used for microfluidic circuit flushing, dissolved at 20 g/L
Custom adapter Custom provider - Custom machined adapter to connect microinjectrode to hydraulic microdrive
Driver LabSmith T7 TORX driver for installing breadboard screws (LS-TORX Driver) https://products.labsmith.com/ls-torx-driver/#.XO8sndNKh25
Epoxy glue LabSmith Two-part high-strength epoxy adhesive (LS-EPOXY) for metal and plastic bonding https://products.labsmith.com/ls-epoxy-12ml-epoxy-adhesive/#.XO8t89NKh24
Ferrule LabSmith One-Piece Fitting (C360-100) for connecting capillary, thru hole sized for 360μm OD capillary https://products.labsmith.com/one-piece-fitting#.XNYEaNNKh24
Ferrule plug LabSmith One-Piece Plug (C360-101) for use in any -C360 port https://products.labsmith.com/one-piece-fitting-plug/#.XNYFl9NKh24
Ferrule wrench LabSmith 1/8” hex wrench for installing one-piece fittings and plugs (LS-HEX 1/8” Hex Wrench) https://products.labsmith.com/ls-hex-1-8-hex-wrench/#.XO8sqtNKh24
Gastight syringe Hamilton Company 500μL gastight syringe model 1750 (81220) and 1mL gastight syringe model 1001 (81320) https://www.hamiltoncompany.com/laboratory-products/syringes/81220#top
Gold pins Aim-Cambridge Male gold plated crimp-on connector pin (40-9856M) https://www.masterelectronics.com/aim-cambridge-cinch-connectivity-solutions/409856m-10109145.html
Lint-free wipes Kimberly Clark Kimtech Science Kimwipes Delicate Task Lint-free wipes, used to identify leaks in the system
Liquid food color McCormick & Co. Water based, black liquid food color (52100581873) https://www.mccormick.com/spices-and-flavors/extracts-and-food-colors/food-colors/black-food-color
Low viscosity oil Clearco Products Co. Pure Silicone Fluid Octamethyltrisiloxane with a viscosity of 1cSt at 25°C (PSF-1cSt) http://www.clearcoproducts.com/pure-silicone-super-low-viscosity.html
Luer-Lock connector LabSmith Luer-Lock Adapter (C360-300), female fitting for connecting Luer Lock syringe to 360μm capillary tubing https://products.labsmith.com/luer-lock-adapter-assembly#.XO81MtNKh24
Micro drill bits Grainger Micro drill bit, 0.23mm (414H85) https://www.grainger.com/category/machining/drilling-and-holemaking/drill-bits/machining-drill-bits/micro-drill-bits
Microelectrode FHC Metal microelectrode, tungsten with epoxy insulation https://www.fh-co.com/category/metal-microelectrodes
Oil hydraulic micromanipulator Narishige Group Oil Hydraulic Micromanipulator with guide tube attached (MO-96) http://products.narishige-group.com/group1/MO-96/chronic/english.html
Polymicro Capillary Tubing Molex Polymicro Flexible Fused Silica Capillary Tubing (TSP150375), Outer Diameter 375μm, Inner Diameter 150µm https://www.molex.com/webdocs/datasheets/pdf/en-us/1068150024_CAPILLARY_TUBING.pdf
Programmable syringe pump Harvard Apparatus Standard Infuse/Withdraw Pump, programmable (70-2213) https://www.harvardapparatus.com/standard-infuse-withdraw-pump-11-pico-plus-elite-programmable-syringe-pump.html
Ruler Empire Stainless steel 6” Stiff ruler (27303) http://www.empirelevel.com/rulers.php
Screw set LabSmith Valve mounting screw set (LS-SCREWS .25), thread-forming screws (2-28 x 1/4”) to mount valves to breadboard https://products.labsmith.com/ls-screws-25#.XO8widNKh24
Standard Breadboard LabSmith 4” x 6” platform (LS600), with 0.25” hole spacing for mounting fluid circuit https://products.labsmith.com/standard-breadboard/#.XO8xDdNKh24
Sterile saline (sodium chloride) 0.9%. Baxter 0.9% Sodium Chloride sterile Sterile Intravenous Infusion
Sterile syringe filters Millipore Sigma MilliporeSigma Millex-GP Sterile Syringe Filters with PES Membrane (SLGPM33RS) https://www.fishersci.com/shop/products/emd-millipore-millex-sterile-syringe-filters-pes-membrane-green-4/slgpm33rs
Stoelting manual microsyringe pump Stoelting Company Manual infusion/withdrawal pump (51222) https://www.stoeltingco.com/manual-infusion-withdrawal-pump-2649.html
T-junction LabSmith Interconnect tee (C360-203) for combining flow streams, for use with 360μm OD capillary tubing https://products.labsmith.com/interconnect-tee#.XO8z8dNKh24