Skip to main content
. Author manuscript; available in PMC: 2020 May 24.
Published in final edited form as: Cancer Cell. 2019 Feb 14;35(3):401–413.e6. doi: 10.1016/j.ccell.2019.01.008

Figure 2. ARv7, Unlike ARfl, Functions as a Transcriptional Repressor in CRPC Cells.

Figure 2.

(A and B) Volcano plots of differentially expressed genes in shARv7 (A) or shARfl (B) cells, compared with shGFP control. Significantly altered genes (fold change >±1.5; adjusted p value <0.05) are highlighted in red (activated) or blue (repressed). Select AR targets and significant outliers are labeled.

(C) Top: violin plots of log2 fold changes of ARv7-regulated (blue) and ARfl-regulated (red) genes (relative to the shGFP control) in response to DHT stimulation. Only genes with an adjusted p value of <0.05 are shown. Bottom: bar plots of the mean log2 fold changes of the ARv7-regulated (blue) and ARfl-regulated (red) target genes as above. ****p % 0.0001 by ANOVA and Tukey’s honest significant difference test (HSD).

(D) Comparison of log2 fold changes of significantly dysregulated genes (adjusted p value <0.05) in response to shARv7 or shARfl (as defined in A and B). Colors indicate genes primarily dysregulated by shARfl (red), shARv7 (blue), or both (purple). Select classical AR targets are labeled.

See also Figure S2; Tables S1 and S2.