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. Author manuscript; available in PMC: 2020 May 24.
Published in final edited form as: Cancer Cell. 2019 Feb 14;35(3):401–413.e6. doi: 10.1016/j.ccell.2019.01.008

Figure 4. ARv7 Binds to Transcriptional Co-repressors NCOR1, NCOR2, and NRIP1.

Figure 4.

(A) MARCoNI assay (using a pan-AR antibody) of select corepressor peptides and cell lysates from indicated LNCaP95 cells. Results are the mean of three experiments ±SD. *p % 0.05, ***p % 0.001, Student’s t test.

(B) Quantification of ARv7:NCOR1,2 coIP signals from nuclear lysates of shGFP, shARv7, and shARfl cells. Values are the means ±SEM of 5–6 replicates (n). n.s., not significant; *p % 0.05,**p % 0.01, ***p % 0.001, paired one-tailed t test.

(C) Heatmap of the union of dysregulated genes (adjusted p value <0.05) in response to NCOR1 or NCOR2 KD (siNCOR1 or siNCOR2) relative to control (siCtrl) in the background of shGFP, shARv7, and shARfl cells.

See also Figure S4.