KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-AR (N-20) | Santa Cruz Biotechnologies | Cat# sc-816, RRID:AB_1563391 |
| Mouse monoclonal anti-AR (441) | Santa-Cruz Biotechnologies | Cat# sc-7305, RRID:AB_626671 |
| Rabbit polyclonal anti-AR (PG21) | Millipore | Cat# 50-172-110 |
| Rabbit polyclonal C-terminal AR (Cone SP242) | Spring Bioscience | Cat# M5422 |
| Rabbit polyclonal C-terminal AR (C-19) | Santa Cruz Biotechnologies | Cat# sc-815, RRID:AB_630864 |
| Rabbit monoclonal ARv7 (H6 253) | Epitomics (now Abcam) | Cat# ab198394 |
| Rabbit monoclonal anti-ARv7 (clone RM7) | RevMab | Cat# 31-1109-00, RRID:AB_2716436 |
| Rabbit polyclonal NCOR1 (PA1-844A) | Thermo Fisher Scientific | Cat# PA1-844A, RRID:AB_2149004 |
| Rabbit polyclonal NCOR2 (ab24551) | Abcam Abcam | Cat# ab24551, RRID:AB_2149134 |
| Rabbit polyclonal anti-H3K27ac (C15410196) | Diagenode | 15410196;RRID:AB_2637079 |
| Rabbit polyclonal FOXA1 (ab23738) | Abcam | Cat# ab23738, RRID:AB_2104842 |
| Goat polyclonal FOXA1 (ab5089) | Abcam | Cat# ab5089, RRID:AB_304744 |
| Mouse monoclonal b-Actin | Abcam | Cat# ab8226 |
| Rabbit monoclonal Lamin B1 (EPR8985) | Abcam | Cat# ab194109 |
| Monoclonal mouse anti-rabbit IgG, light chain specific | Jackson ImmunoResearch | Cat# 211-032-171 |
| FITC-conjugated swine anti-rabbit | DAKO | Cat# F0205 |
| Biological Samples | ||
| CRPC tumor metastases samples | University of Washington and Fred Hutchinson Cancer Research Center | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DHT (5α-androstan-17β-ol-3-one) | Sigma | Cat# A8380 |
| Critical Commercial Assays | ||
| TruSeq stranded mRNA sample kit | Illumina | Ref# 20020594 |
| ThruPLEX DNA-seq kit | Rubicon Genomics | Ref# R400427 |
| Deposited Data | ||
| RNA-seq | This paper | GEO: GSE106560 |
| ChIP-seq | This paper | GEO: GSE106559 |
| CRPC tumor metastases samples | Kumar et al., 2016 | GEO: GSE77930 |
| Genome-wide expression profiling of radical prostatectomies | Den et al., 2014 | GEO: GSE72291 |
| Genome-wide expression profiling of radical prostatectomies | Ross et al., 2016 | GEO: GSE79957 |
| Genome-wide expression profiling of radical prostatectomies | Benzon et al., 2017 | GEO: GSE79956 |
| Genome-wide expression profiling of radical prostatectomies | Karnes et al., 2013 | GEO: GSE62116 |
| Genome-wide expression profiling of radical prostatectomies | Erho et al., 2013 | GEO: GSE46691 |
| Genome-wide expression profiling of radical prostatectomies | Klein et al., 2015 | GEO: GSE62667 |
| Genome-wide expression profiling of radical prostatectomies | Boormans et al., 2013 | GEO: GSE41408 |
| Genome-wide expression profiling of radical prostatectomies | Taylor et al., 2010 | GEO: GSE21032 |
| Taylor dataset | Taylor et al., 2010 | N/A |
| Experimental Models: Cell Lines | ||
| Human: LNCaP95 | Hu et al., 2012 | N/A |
| Human: LNCaP95 shGFP | This paper | N/A |
| Human: LNCaP95 shARfl | This paper | N/A |
| Human: LNCaP95 shARv7 | This paper | N/A |
| Human: 22Rv1 | ATCC | Cat# CRL-2505 |
| Human: 22Rv1 shGFP | This paper | N/A |
| Human: 22Rv1 shARfl | This paper | N/A |
| Human: 22Rv1 shARv7 | This paper | N/A |
| Human: Hep3B | ATCC | Cat# HB-8064 |
| Oligonucleotides | ||
| shGFP-forward: 5’-CCGGGCAAGCTGACCCTGAAGTTCACTCG AGTGAA CTTCAGGGTCAGCTTGCTTTTTG-3’ | This paper | N/A |
| shGFP-reverse: 5’-AATTCAAAAAGCAAGCTGACCCTGAAGTT CACTCGAGTGAACTTCAGGGTCAGCTTGC-3’ | This paper | N/A |
| shARfl-forward: 5’-CCGGCCTGCTAATCAAGTCACACATCTCG AGATGTGTGACTTGATTAGCAGGTTTTTG-3’ | This paper | N/A |
| shARfl-reverse: 5’-AATTCAAAAACCTGCTAATCAAGTCACAC ATCTCGAGATGTGTGACTTGATTAGCAGG-3’ | This paper | N/A |
| shARv7-forward: 5’-CCGGGTAGTTGTGAGTA TCATGACTCGAGTCATGATACTCACAACTACTTTTTG-3’ | This paper | N/A |
| shARv7-reverse: 5’-AATTCAAAAAGTAGTTGTG AGTATCATGACTCGAGTCATGATACTCACAACTAC-3’ | This paper | N/A |
| siCtr_1: 5’-UGGUUUACAUGUCGACUAA-3’ | GE Dharmacon | Cat# D-001810-01-20 |
| siCtr_2: 5’-UGGUUUACAUGUUGUGUGA-3’ | GE Dharmacon | Cat# D-001810-02-20 |
| siNCOR1_1: 5’-GCUGAGGGCUUCUGCAGAU-3’ | GE Dharmacon | Cat# J-003518-06-0002 |
| siNCOR1_2: 5’-GGAAAGUCCUCCCAUACGA-3’ | GE Dharmacon | Cat# J-003518-07-0002 |
| siNCOR2_1: 5’-CAGCCAGGGAAGACGCAAA-3’ | GE Dharmacon | Cat# J-020145-10-0002 |
| siNCOR2_2: 5’-AGGCAUCCCAGGACCGAAA-3’ | GE Dharmacon | Cat# J-020145-11-0002 |
| siNRIP1_1: 5’-GAAGCGUGCUAACGAUAAA-3’ | GE Dharmacon | Cat# J-006686-05-0002 |
| siNRIP1_2: 5’-AGAAGGAUGUUGGCAGUUA-3’ | GE Dharmacon | Cat# J-006686-06-0002 |
| Primers for site-directed mutagenesis of pEYFP-(GA)6-ARv7 (forward): 5’-AGCAAAAATGATTGCA CTATTGAT-3’ | This paper | N/A |
| Primers for site-directed mutagenesis of pEYFP-(GA)6-ARv7 (reverse): 5’-GGCGCACAGGTACT TCTGTTT-3’ | This paper | N/A |
| Recombinant DNA | ||
| pLKO-TET-ON | Addgene | Cat# 21915 |
| LentiCRISPR v2 | Fei et al., 2017 | N/A |
| pEYFP-(GA)6-AR R585K | This paper | N/A |
| pECFP-(GA)6-AR | van Royen et al., 2012 | N/A |
| pEYFP-(GA)6-AR | van Royen et al., 2012 | N/A |
| pEYFP-(GA)6-ARv7 R585K | This paper | N/A |
| pECFP-(GA)6-ARv7 | This paper | N/A |
| pEYFP-(GA)6-ARv7 | This paper | N/A |
| Software and Algorithms | ||
| Aperio ImageScope | Leica Biosystems | N/A |
| Aperio Brightfield Image Analysis Toolbox | Leica Biosystems | N/A |
| SAM | Tusher et al., 2001 | N/A |
| ImageJ | Schneider et al., 2012 | N/A |
| VIPER | Cornwell et al., 2018 | https://bitbucket.org/cfce/viper/ |
| DEseq | Anders and Huber, 2010 | N/A |
| CHIPS 2 | Qin et al., 2016 | N/A |
| Bamliquidator | Loven et al., 2013; Whyte et al., 2013 | N/A |
| SeqPos | He et al., 2010 | N/A |
| MAGeCK | Li et al., 2014 | N/A |