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. 2020 Apr 21;52(5):711–724. doi: 10.1007/s00726-020-02845-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The total levels of amino acids in freshly prepared equine erythrocytes at time 0 and then after 2, 5, 10, and 20 min incubation in pre-warmed AA-PBS (orange, mean ± SE, n = 3). The corresponding levels of amino acids in the external AA-PBS medium were also assessed (blue, n = 3). Significant differences were marked between the initial value and the subsequent time points; **p < 0.001; ***p < 0.0001