Fig. 6.
EPS-37 inhibits mitogen induced and antigen specific T-cell proliferation in vitro. Spleen cells from OT II transgenic (a–c) or C57BL6 mice (d) were stimulated (white bars) in vitro (5 × 105/well, in 0.2 ml) with 100 μg/ml OVA (a, b) or 1 μg/ml OVA 323–339 peptide (OVAp, c), or 1 μg/ml concanavalin A (ConA, d). EPS-37 (black bars) (30 μg/ml; except b 1–100 μg/ml) or ArGal (hashed bars) (30 μg/ml) were added to the culture. 3H-thymidine was introduced into the culture for the last 18 h. The proliferation of cells is shown as stimulation index (CPM of stimulated cell/CPM of unstimulated cells) (a, b, d). IFN-γ (c) content measured in cell culture supernatant is shown in ng/ml. Results of selected experiments, expressed as a mean of triplicates of the culture wells ± SEM are shown. *P < 0.05, **P < 0.01, ***P < 0.001