Fig. 5.
Viral replication of DENV-2 8H2-7-LP and DENV-2 1D4-5-SP in Balb/c mice pretreated with IFNAR blocking antibodies. Female Balb/c mice of 6–8 weeks old were injected through i.p. with 2 mg antibody to type I IFN receptor (MAR1-5A3) at one day prior to infection. On the next day, 106 PFU of viruses were inoculated into mice through s.c. route. Then, blood samples were collected daily after infection. A Viral RNA copies in blood were measured using qRT-PCR, Two-way ANOVA was used in statistical analysis of the differences among groups, **P < 0.01, ****P < 0.0001, and B Titers of infectious virus in serum samples were determined through foci forming assay in Vero cells; n = 5. T test was used in statistical analysis of the differences among groups, *P < 0.05, **P < 0.01, *** P < 0.001, ****P < 0.0001. C Eye, liver and spleen were collected from 8H2-7-LP virus-infected mice, and viral RNA loads were determined, n = 4. Detection limits of RNA copy and infectious virus titer were indicated with dotted lines. N.D. not detected.