Figure 1.

Primary NSC culture and characteristics. (a) Cultured cells exhibited a growth pattern of free floating neurospheres. Scale bars: 100 μm. (b) Immunostaining indicated that most of cultured cells expressed nestin. Scale bars: 20 μm. (c) Immunostaining showed that cultured cells held the potential of differentiation into MAP⁺ cells. Scale bars: 20 μm. (d) Immunostaining demonstrated that cultured cells bore the ability of differentiation into GFAP⁺ cells. Scale bars: 20 μm. (e) Immunostaining delineated cultured cells possessed the capacity of differentiation into Olig2⁺ cells. Cell nuclei were counterstained with DAPI in blue. Scale bars: 20 μm.