Figure 5.

ACTA2 downregulation increased active RhoA and downregulated Rac1 expression. (a) Bands represented the expression of RhoA, Rac1, and CDC42. Total RhoA and GAPDH was used as a loading control. (b) Quantification of active RhoA expression from (a) (n = 3 for each group). ^∗∗∗p < 0.001, one-way ANOVA followed by Tukey's post hoc test. (c) Quantification of Rac1 expression from (a) (n = 3 for each group). ^∗p < 0.05, one-way ANOVA followed by Tukey's post hoc test. (d) Quantification of CDC42 expression from (a) (n = 3 for each group).