Figure 5.

SseK3 interacts with TBCB in the yeast two-hybrid system. Strain L40 of S. cerevisiae was cotransformed with derivatives of plasmids pLEX10, to generate fusions of SseK1, SseK2, or SseK3 with the DNA-binding domain of LexA, and pGAD1318, to generate a fusion of TBCB with the activation domain of Gal4, as indicated. The interaction between the two hybrid proteins is shown by the growth in the absence of histidine (-His), and detection of blue color in the presence of X-Gal after a β-galactosidase filter assay.