Figure 2.

The ketogenic diet (KD) reverses alterations in mitochondrial bioenergetics in BTBR mice. Isolated mitochondria were used for mitochondrial bioenergetics assays with a Seahorse XF24 analyzer. (A) The bar graphs depict the oxygen consumption rates (OCR) under basal conditions and (B) relative ATP production using pyruvate and malate as substrates. (C) Densitometry analysis of P-AMPK ^T172. (D) Representative blots for the densitometry analysis in Figure 2C. (E) Relative basal respiration levels via Complex II, after the addition of ADP (after subtraction of antimycin A-insensitive OCR (non-mitochondrial respiration)). The enzyme activity (F) and protein expression levels (G) of electron transport chain Complex II. Data are shown as mean±SEM, n = 6 per group. Data were analyzed by two-way ANOVA. The significant effects (main effects from two-way ANOVA) are presented as: ### p<0.001. Further, the significant differences between groups revealed by the post-hoc analysis, are presented as: *** p<0.001; ** p<0.01.