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Validating the stemness of human adipose MSCs. (A) Adipogenic, osteogenic, and chondrogenic differentiating abilities of human adipose MSCs were validated by via Oil-red O (adipogeneis), Alizarin Red (osteogenesis), and Safranin O (chondrogeneis) staining, respectively. (B) Flourescence activated cell sorting (FACS) analysis of human adipose MSCs using positive (CD73, CD90, CD105) and negative (HLA-DR, CD14, CD34, CD45, and CD11b) markers. Scale bars = 200 µm.
