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. 2020 Apr 26;21(9):3052. doi: 10.3390/ijms21093052

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Persistence of human MSCs in the xenogeneic MSC group. (A) Based on immunohistochemical staining of anti-human STEM 121, barely any viable human MSCs were detected around the injection site seven days after injection. Red frames indicated magnified area of the injection sites and STEM 121-positive cells are indicated by solid yellow arrows. (B) Alu PCR quantification of remaining human adipose MSCs at 0 h and seven days post transplantation is shown on the right. Very few MSCs are present by seven days after MSC injection. Statistical significance was defined as *** p < 0.001 vs. 0 h; mean ± S.E.M. Scale bars: whole brain = 2 mm, magnified image: 50 µm.