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. 2020 May 6;21(9):3287. doi: 10.3390/ijms21093287

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of media conditioned by senescent cells on platelet activation. (A) The presence of activated GPIIb/IIIa and P-selectin on the surface of platelets, previously exposed to media conditioned by senescent and non-senescent HMEC-1 cells, was determined by flow cytometric analyses. Platelets under basal and sub-aggregating (ADP sub) conditions were tested. (B) Representative dot plots for the detection of CD61 (platelets; top), PAC-1 (activated GPIIb/IIIa; middle) and CD62 (P-selectin; bottom) on human platelets. ADP sub: 1.3–2.0 μM ADP; C-CM: Control conditioned medium; S-CM: Senescent conditioned medium; SSC: side scatter; FSC: forward scatter. The graph depicts the mean ± SD of n = 4 (PAC-1) and n = 7 (CD62) experiments. * p < 0.05 and *** p < 0.001 analyzed by Student’s t-test.