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. 2020 Apr 26;21(9):3050. doi: 10.3390/ijms21093050

Figure 2.

Figure 2

TCS induced reactive oxygen species (ROS) and superoxide production and activated mitochondrion-related antioxidant enzymes in matured porcine COCs. (a) Detection of intracellular ROS levels in porcine COCs after TCS treatment at different concentrations (1, 10, and 100 μM) using dichlorofluorescein diacetate (DCF-DA) (green fluorescence) staining. Scale bar = 100 µm. (b) Identification of mitochondrion-specific superoxides using Mito-SOX staining (red fluorescence) following exposure of COCs to TCS. Mito-SOX (red)-stained cytoplasm was examined using the iRiS™ Digital Cell Image System. Scale bar = 100 µm. (c) mRNA levels of mitochondrion-related antioxidant enzymes (Sod2, Prdx3, and Prdx5) in maturing porcine COCs were measured using RT-PCR, and Gapdh was used as an internal control. Histograms represent the values of densitometry analysis obtained using ImageJ software. Data in the bar graph are presented as the mean ± SEM of three independent experiments (per 30 COCs). Differences were considered significant at * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the non-treated group.