Table 5.
Primer sequences of cumulus expansion factors, mitochondria-related antioxidant enzymes, and apoptosis factors from matured COCs for RT-PCR.
| Genes | Primer Sequences | Tm °C | Gene Bank Accession No. |
Base Pairs |
|---|---|---|---|---|
| Has2 | F(5′–3′): TGGCTGTACAATGCGATGTG R(5′–3′): TGGGTGGTGTGATTTTCACC |
55 | (NM_214053.1) | 402 |
| Tnfaip6 | F(5′–3′): TCTTCCTGTGGGAAGAGGCT R(5′–3′): GTCCGTCTGAACAGAAGCGA |
55 | (NM_001159607.1) | 337 |
| Ptx3 | F(5′–3′): TCAGTGCCTGCATTTGGGTC R(5′–3′): TTCTGAACAAGGGCATGTAG |
58 | (NM_001244783.1) | 225 |
| Sod2 | F(5′–3′): GCAGCTCGAGCAGGAATCTGG R(5′–3′): ACGCGGCCTACGTGAACAA |
59.7 | (NM_214127.2) | 163 |
| Prdx3 | F(5′–3′): AGTGGATTCCCACTTCAGCC R(5′–3′): AACCCATGGAGAAGTCTGCC |
55.1 | (NM_001244531.1) | 290 |
| Prdx5 | F(5′–3′): ACCTTCCAGGGTTTGTGGAG R(5′–3′): CCTGAATGTGGAGCCAGATG |
55 | (NM_214144.1) | 285 |
| Bax | F(5′–3′): AAGCGCATTGGAGATGAACT R(5′–3′): CTGGACTTCCTTCGAGATCG |
50 | (XM_003127290.4) | 251 |
| Bcl-xl | F(5′–3′): AGGGCATTCAGTGACCTGAC R(5′–3′): CACCTAGAGCCTTGGATCCA |
55 | (NM_214285.1) | 242 |
| Caspase3 | F(5′–3′): GAGGCAGACTTCTTGTATGC R(5′–3′): TTCCATGTATTGTGTCCATGC |
50 | (NM_214131.1) | 238 |
| Gapdh | F(5′–3′): TCGGAGTGAACGGATTTC R(5′–3′): CCTGGAAGATGGTGATGG |
53.7 | (NM_001206359.1) | 230 |
Has2; HA synthase 2, Tnfaip6; TNF-a-induced protein 6, Ptx3; Pentraxin 3, Sod2; Superoxide dismutase 2, Prdx3; Peroxiredoxin 3, Prdx5; Peroxiredoxin 5, Bax; Bcl2-associated X protein, Bcl-xl; B-cell lymphoma-extra large, Gapdh; Glyceraldehyde-3-Phosphate Dehydrogenase, Tm; Meting Temperature, F; Forward, R; Reverse.