Figure A3.

Western blot analysis of glucose transporters (Glut1, Glut2) and glucokinase (Gck) in pancreatic tissue analysing male and female wild-type (Wt) mice and cannabinoid receptor knockout mouse lines (CB₁^−/−, CB₂^−/−) separately. (a) Glut1 seemed to be decreased in CB₁^−/− and CB₂^−/− knockout mice of both sexes. (b) Glut2 protein was significantly decreased in CB₁^−/− and CB₂^−/− knockout mice of both sexes. (c) Gck protein level was increased in female CB₁^−/− mice. Analysis of male and female mice was done separately on different immunoblots, defining male and female Wt protein levels as 100%. A representative western blot of each protein is shown in the right column showing specific single bands as indicated. Vinculin (Vinc) was used as control protein. Values are presented as mean (±S.E.M.) with n = 4–9 mice per group. * p < 0.05; ** p < 0.01 for comparisons within male or female Wt and knockout mice; unpaired t-test.