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. 2020 May 5;21(9):3265. doi: 10.3390/ijms21093265

Figure 1.

Figure 1

Effects of bee venom (BV), melittin (MLT), apamin, and phospholipase A2 (PLA2) on the cell viability and LDH release in iPSCs and iPSCs-Diff. (A) iPSCs and iPSCs-Diff were seeded on 24-well culture plates and then treated with the indicated concentrations of BV, MLT, apamin, and PLA2. After 24 h, viable cells were determined after crystal violet staining. Relative cell viability compared to untreated cells was calculated and expressed as means ± SD from triplicate samples. (B) iPSCs and iPSCs-Diff were treated with the indicated concentrations of BV, MLT, apamin, and PLA2 for 24 h and then LDH levels in culture supernatants were measured. The percentage LDH release calculated based on the background control and high control was expressed as means ± SD from triplicate samples. (C) iPSCs and iPSCs-Diff seeded in 12-well culture plates were incubated in the presence of BV at 1, 2.5, and 5 μg/mL. At 24 h post-treatment, cells were photographed under an inverted microscope. Scale bar = 100 μm.