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. 2020 May 4;21(9):3254. doi: 10.3390/ijms21093254

Figure 3.

Figure 3

Chronic exposure to low-dose rotenone induced cholinergic neurodegeneration in the dorsal motor nucleus of the vagus (DMV) of mice. (A) Representative photomicrographs of immunohistochemistry for choline acetyltransferase (ChAT) in the DMV of mice 4 weeks after rotenone treatment. Scale bar = 100 µm. CC: central canal, X: DMV, XII: hypoglossal nucleus. Arrowheads: Vagal nerve. (B) Changes in the number of ChAT-positive neurons in the DMV. Each value is the mean ± SEM (n = 4). * p < 0.05 vs. the vehicle-treated control group (two-tailed unpaired independent t-test).