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. 2020 May 4;21(9):3255. doi: 10.3390/ijms21093255

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) throughout the incubation of sperm in capacitating medium (control samples) and capacitating medium plus 1 mM, 5 mM or 10 mM 2-GBI. The blocker was added to capacitating medium either at 0 min in experiment 1 (A–C) or after 240 min of incubation in experiment 2 (D–F; acute). Different superscript letters (a–c) indicate significant differences (p < 0.05) between samples within the same time point, and different superscript numbers (1–3) indicate significant differences (p < 0.05) between time points within a given treatment. Results are expressed as mean ± SEM (n = 10). The arrow indicates the time at which 10 µg/mL of progesterone was added to all samples (i.e., 240 min of incubation).