Figure 10.

Percentages of viable sperm with high intracellular calcium levels evaluated through Rhod5 (Rhod5⁺) in relation to total viable sperm (YO-PRO-1⁻), and fluorescence intensity of Rhod5⁺ in viable sperm throughout incubation in capacitating medium (control samples) or capacitating medium plus 1 mM, 5 mM or 10 mM, added at either 0 min in experiment 1 (A,B) or 240 min in experiment 2 (C,D; acute). Different superscript letters (a–c) indicate significant differences (p < 0.05) between samples within the same time point, and different superscript numbers (1–4) indicate significant differences (p < 0.05) between time points within a given treatment. Results are expressed as mean ± SEM (n = 10). The arrow indicates the time at which 10 µg/mL of progesterone was added to all samples (i.e., 240 min of incubation).