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. 2020 Apr 26;21(9):3049. doi: 10.3390/ijms21093049

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Western blot analysis of oocytes treated by 4-(imino)-1,4-dihydroquinolines. Oocytes untreated (C) or treated with progesterone (PG) were submitted to 4-(imino)-1,4-dihydroquinolines 6, 7, 8, 9, 10, 11 for 24 h. After electrophoresis, antibodies were used to reveal total and phosphorylated forms of Erk2 and P-Erk2 on Y202/Y204, Rsk and P-Rsk on S380, Cdk1 and P-Cdk1 on Y15, and phosphorylated form of P-H3 on S10, P-H2B on S14, or cleaved caspase 3, cyclin B2, Mos and actin. The membranes were revealed using an Advanced ECL chemiluminescence technology. Staurosporine 20 mM was used as apoptosis control. Erk2, Rsk, Cdk1 and actin serve as internal loading controls.