Figure 2.

Effect of rapamycin in aortic tissue. Immunofluorescence staining of the aortic tissue for Ki67 and SMA (A) or CD45 (B) with nuclear 4’,6-diamidino-2-phenylindole (DAPI) staining, before (Pre) or after 3 days of BAPN + AngII challenge with vehicle (BA+DMSO) or rapamycin (BA+Rapa) treatment. Representative immunofluorescence images (upper panels) and scattergrams of imaging cytometry (bottom panels) are shown. The percentages of cell populations are indicated in the scattergrams of the quadrants as arbitrarily determined by the thresholds for the signal intensities of Ki67 (A,B), SMA (A) and CD45 (B). The thresholds are set constant for Pre, BA+DMSO, and BA+Rapa in a given double staining. Scale bars: 50 µm.