Figure 1.

COX-2 fragments in cell lines and human samples. (A) 100 μg of total tissue homogenates of brain samples of glioblastoma multiforme (BGM) models of transgenic mice were resolved by SDS-PAGE and probed for the presence of COX-2. (Neuro-tumor from neuronal origin, Astro- from astrocyte origin and Neuro SC-from neural stem cells). Black arrowhead marks the full-length (F.L.) COX-2 and the red arrowheads mark the lower fragments. (B) Samples of tumors (T) and surrounding seemingly healthy tissues (N = Normal) obtained from four patients diagnosed with colorectal adenocarcinoma were probed for COX-2 expression. Blots show the presence of several immunoreactive bands, some of which are lower than the expected MW of the native protein (Red arrows).