Figure 1.

Effect of tumor growth factor beta (TGFβ) induction to migration behavior of keratinocytes. Cells cultured in 12-well plates were mechanically scratched with a sterile 10 µL pipette tip and then allowed to re-epithelialize for 24 h at 37 °C in the presence or absence of TGFβ. (A) The bar represents measurements of wound closure expressed as the percentage of initial wound area at 5, 10, 15, and 20 h. (B) The bar graph represents rate of wound healing derived from the wound area measurements at 5, 10, 15, and 20 h; (C) Representative images of scratch wound; (D) The histogram represents distribution of keratinocytes that migrates at 0–50, 50–100, 100–150, and <150 µm/h velocity at 5, 10, 15, and 20 h; (E) The average migration velocity of keratinocytes at all time points. (F) The average directionality of keratinocytes migration at all time points. The * indicate significant differences with p < 0.05 according to student t-test analysis.