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. 2020 May 6;17(9):3229. doi: 10.3390/ijerph17093229

Figure 2.

Figure 2

Effect of TGFβ induction to endpoint outcome of epithelial to mesenchymal transition (EMT). Cells cultured in 6-well plates in the presence or absence of TGFβ were analyzed for circularity parameters and EMT markers expression. (A) Representative images of keratinocytes cultured in the presence or absence of TGFβ; (B) Quantitative analysis of the circularity index of keratinocytes cultured in the presence or absence of TGFβ; (C) Representative fluorescence images of keratinocytes cultured in the presence or absence of TGFβ stained with antibodies against E-cadherin and vimentin; (D) Quantitative analysis of the relative intensity of E-cadherin and vimentin expression of keratinocytes cultured in the presence or absence of TGFβ. The * indicate significant differences with p < 0.0001 according to student t-test analysis.