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. 2020 May 6;17(9):3229. doi: 10.3390/ijerph17093229

Figure 4.

Figure 4

Effect of KH on the TGFβ-induced migration behavior of keratinocytes. Cells cultured in 12-well plates were mechanically scratched with a sterile 10 µL pipette tip and then allowed to re-epithelialize for 24 h at 37 °C with KH treatment in the presence or absence of TGFβ. Cells cultured without KH or TGFβ were analyzed as negative control. (A) The bar represents measurements of wound closure expressed as the percentage of initial wound area at 5, 10, 15, and 20 h. (B) The bar graph represents rate of wound healing derived from the wound area measurements at 5, 10, 15, and 20 h; (C) Representative images of scratch wound; (D) The histogram represents distribution of keratinocytes that migrates at 0–50, 50–100, 100–150 and <150 µm/h velocity at 5, 10, 15, and 20 h; (E) The average migration velocity of keratinocytes at all time points; (F) The average directionality of keratinocytes migration at all time points. The * indicate significant differences with p < 0.05 according to student t-test analysis.