Figure 5.

Effect of KH on TGFβ-induced EMT. Cells cultured in 6-well plates treated with KH in the presence or absence of TGFβ were analyzed for circularity parameters and EMT markers expression. Cells cultured without KH or TGFβ were analyzed as a negative control. (A) Representative images of keratinocytes cultured in different conditions; (B) Quantitative analysis of the circularity index of keratinocytes cultured in different conditions; (C) Representative fluorescence images of keratinocytes cultured in different conditions stained with antibodies against E-cadherin and vimentin; (D) Quantitative analysis of the relative intensity of E-cadherin and vimentin expression of keratinocytes cultured in different conditions. The * indicate significant differences with p < 0.0001 according to student t-test analysis.