Figure 2.

Amuc_1434* treatment induced G0/G1-phase cell-cycle arrest. (A) Cell cycle analysis. (a) LS174T cells were treated with Amuc_1434*, and cell-cycle distribution was evaluated by flow cytometry. (b) Percentages of G0/G1 phase of the cell cycle in LS174T cells are shown. (Data are expressed as mean ± standard deviation, n = 3, *: p < 0.05.) (B) Western blotting analysis for the expression level in LS174T cells of tumor protein 53 (p53) (a), which controls the start of the cell cycle, after treatment with Amuc_1434*. GAPDH was used as the loading control. (b) Quantification of p53 expression levels in LS174T cells. (Data are expressed as mean ± standard deviation, n = 3, *: p < 0.05.).