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. 2020 May 11;21(9):3385. doi: 10.3390/ijms21093385

Figure 4.

Figure 4

Amuc_1434* promoted a change of cellular redox status and mitochondrial dysfunction in LS174T cells. LS174T cells were treated with the indicated concentrations of Amuc_1434* for 24 h. (A) Reactive oxygen species (ROS) production was examined by flow cytometric analysis (a) with 2,7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA) staining, (b) mean fluorescence intensity of ROS for DCFH-DA. Data are expressed as the mean ± standard deviation of three separate experiments, *: p < 0.05; **: p < 0.01. (B) Mitochondrial membrane potential (Δ ψ) was detected by fluorescence microscopy using JC-1 staining. (a) Fluorescent micrographs of mitochondrial membrane potential obtained in Amuc_1434*-treated LS174T cells at 24 h, bar = 500 μm; (b) Quantification of relative fluorescent intensity per cell determined by Image J software. Values represent as ratio of red to green fluorescence (**: p < 0.01, n = 3). Data are mean ± S.E.M.