Effects of Fe, Mn, Cu and Zn on cucumber plants under fusaric acid (FA) supply. (A) 3-week-old seedlings were cultured with Hoagland solutions containing 2 µg mL−1 FA and different concentrations of Fe, Mn, Cu or Zn. CK, control plants; CK + FA, control plants after FA treatment. Each ion consists of four concentration series, denoted by 1, 2, 3, 4; Zn concentrations were 0, 9.6, 48 and 144 µM; Cu concentrations were 0, 2, 20 and 40 µM; Mn concentrations were 0, 57, 114 and 570 µM; Fe concentrations were 0, 89.5, 179 and 358 µM. The two treatments in the red box were selected for the following experiments. Thermal images of cucumber plants under light conditions after different treatments were shown on the right. The treatments directly correspond to the left. The temperature quantification results represent the means of four replicates (°C). (B) FA total amount in the plant with different treatments. The letters in the thermal image on the right side of figure (A) indicate statistically significant differences (p < 0.05) among all treatments (a total of 18 treatments). The letters above the bars of the histogram in figure (B) indicate statistically significant differences (p < 0.05) among all different treatments (a total of 17 treatments) and bars represent standard deviation with four replications.