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. 2020 May 11;21(9):3386. doi: 10.3390/ijms21093386

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mobilization of intracellular Ca²⁺ by intense nanosecond pulsed electric field (nsPEF) in CHO (A) and BPAE (B) cells. The extracellular solution did not contain any added Ca²⁺ or Ba²⁺ and was supplemented with 0.5 mM EGTA (CHO) or 1 mM EGTA (BPAE). The electric field strength (kV/cm) for a single 300-ns pulse is indicated in the labels. See Figure 3 and text for more details.