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The different time course of fluorescence in Calbryte-loaded CHO cells during 30 min after nsPEF in the medium with 2 mM Ca2+ (A,B) or Ba2+ (C). Images were taken once a minute. A single 300-ns pulse at 0 (sham exposure), 12.1, or 19 kV/cm was delivered at 122 s. Panels A and B show the same data, with an expanded vertical scale in B. Dashed lines are the polynomial (B) and linear (C) fits through the last 10–15 data points. Mean ± s.e., n = 22–38.
