FIG. 2.

Focused binding of ERα and RNA Pol II on an ERE hotspot in Sμ in estrogen-supplemented B cell cultures. ChIP-seq libraries were prepared from purified B cells stimulated with LPS or LPS plus estrogen (LPS + E). In the latter case, both ERα and RNA Pol II were tested. Potential ERE (RRYYRNNNTGANY) were mapped using the IGV “Find Motif” function. ERE were identified in forward (blue ticks and arrows) and reverse (REV, red ticks) directions. The position of Sμ is indicated by a horizontal blue bar. Data ranges were 0–150 for the LPS library with ERα, 0–106 for the LPS + E library with ERα, and 0–233 for the LPS + E library with RNA Pol II. ERE, estrogen response element.