FIG. 3.

(A) Representative autoradiograms showing [¹⁸F]DCFPyL binding (B_t:[¹⁸F]DCFPyL) and non-specific binding (B_nsp: [¹⁸F]DCFPyL+DCFPyL (10⁻⁶M)) to PSMA in the SMGs of various species. (B, C) Binding affinity (K_d, nM) of [¹⁸F]DCFPyL (B) and PSMA concentrations (C; B_max, nM) in submandibular glands of various species determined from in vitro autoradiograph saturation plots. Each bar represents mean ± SD, n = 3; (D) Representative immunofluorescence staining of PC3-PSMA tumor (i), human submandibular glands (ii), baboon submandibular glands (iii), and mouse submandibular glands (iv) with PSMA antibody (PSMA expression, pink) and DAPI (nuclear stain, blue). SMG, submandibular gland; PSMA, prostate-specific membrane antigen.