Figure EV2. Correlation between single mutant (HO) fitness in proliferation and survival in quiescence.

1. Weak correlation between fitness in proliferation and survival in quiescence under three nutrient‐restricted conditions. The distributions of fitness and survival for thousands of mutants in different conditions are shown on the top (fitness) and right (survival). The Pearson correlation score is on the bottom left of the plot with P < 0.05. All scatter plots, frequency plots, and labels are colored based on media types (orange—carbon restriction, green—nitrogen restriction, and purple—phosphorus restriction).
2. The proportion of mutants with different fitness and survival compared to wild type in proliferation (Pro) and quiescence (Qui) across three nutrient restrictions. Proportions were calculated based on the statistics summarized from linear regression modeling. Better than wild type—regression coefficients—of those mutants are larger than 0 with corrected P < 0.05; no difference compared to wild type are the mutants with corrected P‐value of regression coefficient > 0.05; worse than wild type are the mutants whose regression coefficient is < 0 with corrected P < 0.05.
3. Enriched GO terms identified using Gene Set Enrichment Analysis (GSEA). GSEA was applied to a ranked gene list based on the fitness for proliferative cells and survival for quiescent cells estimated by linear regression modeling. The false discovery rate (FDR) was set at 0.05. Positive enrichment scores (red) indicate functions that have increased fitness or survival relative to WT control. Negative enrichment scores (blue) indicate functions that when impaired result in decreased fitness or survival. Set size indicates the gene number in each enriched term.
4. The 8 genes defined in Fig 2C and their corresponding phenotypic readout in different conditions and cellular states.