Figure 1.

Gnb5 knockout inhibits stimulated insulin secretion in MIN6 cells. Gnb5^+/+ (gray columns) or Gnb5^−/− (open columns) MIN6 cells were stimulated with 10 μm latrunculin B (Lat), 100 μm Oxo-M (Oxo), or both in the presence of 3 mm (3G, A) or 16.7 mm glucose (16G, B). The supernatants were collected for insulin ELISA analysis (y axis). The data points show raw data from five independent cell culture experiments; each black dot is the average of ELISA readings from triplicate wells. Error bars show mean value with standard deviations. To avoid clutter, statistical analysis of the high glucose response is presented in Fig. 2.