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. 2020 Apr 13;295(21):7274–7288. doi: 10.1074/jbc.RA119.011139

Figure 2.

Figure 2.

A: effects of MAAs on the secretion level of HA by HDFs in culture. MAAs at 0, 1, 10, or 25 μg/ml were added to cultures of HDFs for 72 h as noted, and the conditioned medium was measured for HA levels by ELISA. Data represent means ± S.D., n = 4; **, p < 0.01 versus control (0 μg/ml). B: effects of MAAs on the viability of HDFs in culture. HDFs were cultured for 72 h in the presence of MAAs at the indicated concentrations, and their viability was evaluated using the MTT assay. **, p < 0.01 versus control (0 μg/ml). Data represent means ± S.D. n = 4. C–: effects of MAAs on DNA synthesis (C) and collagen 1 (D), and elastin (E) mRNA levels in HDFs. DNA synthesis was measured 48 h after incubation with MAAs at the indicated concentrations using a cell proliferation ELISA kit. Collagen and elastin mRNA levels were measured by qRT-PCR 3 h after incubation with MAAs at the indicated concentrations. Data represent means ± S.D., n = 4.