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. 2020 Apr 13;295(21):7274–7288. doi: 10.1074/jbc.RA119.011139

Figure 7.

Figure 7.

Effects of transfecting an ATF2 siRNA or a c-Fos siRNA. A: effect of transfecting an ATF2 siRNA on the expression of ATF2 protein and its phosphorylation. HDFs were incubated with or without MAAs at 25 μg/ml. Lysates were harvested at 15 min post-treatment and were immunoblotted with antibodies to β-actin and to phosphorylated or nonphosphorylated ATF2. Representative immunoblots from three independent experiments are shown. Data represent means ± S.D., n = 3; **, p < 0.01. B: effect of transfection of a c-Fos siRNA on the MAA-stimulated expressions of c-Fos protein. HDFs were incubated with or without MAAs at 25 μg/ml. Lysates were harvested at 2 h post-treatment and were immunoblotted with antibodies to β-actin and c-Fos. Representative immunoblots from three independent experiments are shown. Data represent means ± S.D., n = 3; *, p < 0.05; **, p < 0.01. C: effects of transfection of an ATF2 siRNA or a c-Fos siRNA on MAA-increased gene expression of HAS2 in HDFs. After transfection of an ATF2 siRNA or a c-Fos siRNA, HDFs were cultured in the presence or absence of MAAs at 25 μg/ml for 3 h and then were subjected to RT-PCR analysis for mRNA levels of HAS2. Data represent means ± S.D., n = 3; *, p < 0.05; **, p < 0.01.