Figure 1.

REDD1 deletion enhances Nrf2 activity and protein expression independent of a change in Nrf2 mRNA abundance. A, the abundances of mRNAs encoding NQO1, GCLC, GCLM, and Nrf2 were evaluated in retinal lysates from wildtype (WT) and REDD1 knockout (KO) mice by PCR. B, Nrf2 activity was measured in the nuclear fraction obtained from retinal lysates by DNA-binding ELISA. C-F, REDD1 CRISPR knockout (sgREDD1) was performed in MIO-M1 cell cultures. C, the abundances of mRNAs encoding NQO1, GCLC, and GCLM was evaluated in cell lysates by PCR. D, Nrf2 activity was measured in cells expressing an ARE luciferase reporter. E, Nrf2 and Lamin B1 protein expression were evaluated in whole cell lysates (WCL) or nuclear isolates by Western blotting. Protein molecular mass (in kilodaltons) is indicated at the right of blots. F, Nrf2 mRNA abundance was assessed in cell lysates by PCR. Values are mean ± S.D. *, p < 0.05 versus WT.