Figure 1.

ATP6V0C co-immunoprecipitates with Vpu. 293T cells were transfected with vectors expressing FLAG-tagged ATP6V0C or Vpu alone or in combination. Twenty-four h post-transfection, cell lysates were prepared and immunoprecipitated (IP) with agarose beads conjugated with anti-FLAG (A) or anti-Vpu (B) antibodies. Complexes were washed, and both cell lysates and immunoprecipitated samples were subjected to immunoblotting with anti-FLAG and anti-Vpu antibodies to detect FLAG-tagged ATP6V0C and Vpu, respectively.