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. 2020 Apr 14;295(21):7327–7340. doi: 10.1074/jbc.RA120.013280

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Figure 3. — Overexpression of ATP6V0C increases the levels of tetherin but reduces CD4 and TR expression. 293T cells were transfected with vectors expressing HA-tagged tetherin without or with FLAG-tagged ATP6V0C and Vpu expression vectors (A), CD4 without or with FLAG-tagged ATP6V0C and Vpu expression vectors (B), HA-tagged tetherin without or with FLAG-tagged ATP6V0C or ATP6V0C″ expression vectors (C), or TR without or with FLAG-tagged ATP6V0C or ATP6V0C″ expression vectors (D). Twenty-four h post-transfection, cell lysates were prepared and subjected to Western blot analysis with anti-FLAG to detect FLAG-tagged ATP6V0C, anti-HA to detect HA-tagged tetherin, or antisera against Vpu, CD4, or TR. Molecular mass markers are shown on the right of the blots.