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. 2020 Apr 14;295(21):7327–7340. doi: 10.1074/jbc.RA120.013280

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Figure 7. — Stabilization of tetherin is not due to inhibition of lysosomal degradation. 293T cells were transfected with vectors expressing HA-tagged tetherin (A) or Vpu (B) without or with FLAG-tagged ATP6V0C expression vector. Eight h post-transfection, cells were either untreated or treated with bafilomycin for 18 h prior to cell lysis and immunoblotting with anti-HA to detect HA-tagged tetherin, anti-FLAG to detect FLAG-tagged ATP6V0C, or anti-TR or anti-tubulin antisera. Molecular mass markers are shown on the right of the blots.