Table 1.
Typical proportion (percentage of total) of the different species purified from 293-F tissue culture supernatant after separation of multimeric species by SEC, as determined by area under the curve analysis using version 7 Unicorn software
Cell culture–derived HMW | Monomer | Assembled HMW | Residual monomer after assembly | |
---|---|---|---|---|
Δ123 | <5 | 47–51 | 60–72 | 28–40 |
Δ123A7 | <1 | 95–100 | 56–70 | 29–44 |
RBD | <1–8 | 22–34 | 40 | 50 |
RBDA7 | <5 | 91–97 | 10 | 90 |