Figure 2.
1H-13C SOFAST-HMQC spectra of α1A-AR-A4. A, overlay of 2D 1H-13C SOFAST-HMQC spectra for ([13CϵH3]Met-) α1A-AR-A4 collected in the apo state (red) and bound to prazosin (black, inverse agonist), WB-4101 (orange, inverse agonist), phentolamine (purple, inverse agonist), silodosin (blue, neutral antagonist), oxymetazoline (cyan, partial agonist), and adrenaline (green, full agonist). B, close-up of the Met-2926.55 resonance. C, close-up of the Met-2035.57 resonance. D, close-up of the Met-1153.41 resonance. Spectra were acquired on ∼50 μm α1A-AR-A4 dissolved in 0.02–0.1% DDM micelle, pH 7.5, and 25 °C.