Figure 6.

G185R ELANE decreased granulocytic differentiation inducers and markers. A–C, the differentiation markers Elane, Mpo, Ltf, Csf3r, and Gr-1 (A); the differentiation transcription factors Gfi1, Spi1, Cebpd, and Cebpe (B); and Cebpa and Cebpe (C) in 32D cells expressing ELANE (WT and G185R) treated with 100 ng/ml GCSF and 1 μg/ml doxycycline for 6 days. The expression levels for each day in cells expressing mutant ELANE are normalized to expression on the respective day in cells expressing WT ELANE. The data are represented as mean ± S.D. of n = 4. Statistical significance was computed using two-way ANOVA followed by multiple comparisons post-test using the Bonferroni method (*, p < 0.05; **, p < 0.01; ***, p < .001; ****, p < 0.0001).