HMGEC	Human meibomian gland epithelial cells
CE	Cholesteryl ester
WE	Wax ester
PC	Phosphatidylcholine
SM	Sphingomyelin
EDTA	Ethylenediaminetetraacetic acid
CM	Chloroform–methanol
TC	Trypsin-containing
nc	Carbon number
KSFM	Keratinocyte serum free media
BPE	Bovine pituitary extract
DMEM	Dulbecco’s modified eagle’s medium
EGF	Epidermal growth factor
FBS	Fetal bovine serum
PTFE	Polytetrafluoroethylene