Figure 2.

Combination treatment using photodynamic therapy and chemotherapy in a 3D compressed collagen cancer construct. The well containing Type 1 collagen hydrogel undergoes compression using an absorber to produce a higher density compressed collagen construct with a much lower water content. The photosensitiser (green) and chemotherapeutic agent (orange) are applied onto the construct and allowed to incubate before washing. The construct is then illuminated with light which results in generation of ROS (yellow).