Figure 5.

Percentage viabilities of SKOV3 (A) and HEY (B) cells in 3D compressed collagen constructs following PDT, dactinomycin only and combination treatments. The Constructs were treated with TPPS2a (0.3 μg/mL for SKOV3 cells) and (0.4 μg/mL for HEY cells), 1 nM dactinomycin and a combination of both drugs. The cultures were treated using a blue lamp at wavelength of 420 nm and light power of 7 mW/cm². The total light doses used were 1.26 J/cm² (3 min), 2.1 J/cm² (5 min) and 2.94 J/cm² (7 min). The cultures were incubated for 48 h post illumination before terminating the experiment. The results were obtained using Alamar Blue assay. Statistical analysis was carried out using 2-way ANOVA. *** p < 0.001. The p values show the significance difference between PDT and combination treatments.