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. 2020 Apr 30;21(9):3203. doi: 10.3390/ijms21093203

Figure 7.

Figure 7

Live-Dead images of SKOV3 3D cultures post PDT (0.3 μg/mL) (B,F,J,N), dactinomycin only (1 nM) (C,G,K,O) and combination treatment (D,H,L,P) using different light conditions, and (A,E,I,M) without light. The cultures were treated using a blue lamp at wavelength of 420 nm and light power of 7 mW/cm2. The total light doses used were 1.26 J/cm2 (3 min), 2.1 J/cm2 (5 min) and 2.94 J/cm2 (7 min). Live-Dead assay was applied 48 h after illumination. To stain the live (green) and dead (red) cells, the 3D constructs were incubated with a solution containing Calcein-AM (live) and Ethidium homodimer-1 (dead). The scale bar presented in each image is 400 µm.